Combination of food wastes for an efficient production of nisin in realkalized fed-batch cultures

Biochemical Engineering Journal

https://doi.org/10.1016/j.bej.2017.03.012

Nisin production by Lactococcus lactis CECT 539 was studied in four realkalized fed-batch cultures in diluted whey with feeding with lactose- and glucose-containing substrates. The first and third cultures were fed with mixtures of whey (W) and a 400 g/L concentrated glucose (CG), or with a concentrated mussel processing waste (CMPW) and CG, respectively.

The second and fourth cultures were respectively performed under the same conditions as in the first and third fermentations. However, these cultures were supplemented with mixtures of W plus a 2% (w/v) yeast extract (WYE2) and CG (second culture), or with CMPW plus a 2% (w/v) yeast extract (CMPWYE2) (fourth culture) after sample extractions at 132 and 168 h, respectively. From these times, each culture was fed with mixtures of WYE2 and CG, or CMPWYE2 and CG, respectively.

The final concentrations of nisin obtained in the third (223.98 BU/mL) and fourth (350.61 BU/mL) cultures, fed with glucose-containing substrates (CG and CMPW), were considerably higher than those obtained in the first (108.00 BU/mL) and second (158.53 BU/mL) cultures fed with the mixture of lactose- and glucose-containing substrates (W and CG).

Effect of crushed mussel shell addition on bacterial growth in acid polluted soils

Applied Soil Ecology, 85 (2015) 65-68

doi:10.1016/j.apsoil.2014.09.010

We applied three different doses of crushed mussel shell (CMS) on two Cu-polluted acid soils to study the effect of these amendments on the growth of the bacterial community during 730 days. Soil pH increased in the short and medium term due to CMS addition. In a first stage, bacterial growth was lower in the CMS-amended than in the un-amended samples. Thereafter, bacterial growth increased slowly. The soil having the highest initial pH value (4.5) showed the first significant increase in bacterial growth 95 days after the CMS amendment. However, in the soil with the lowest initial pH value (3.8) bacterial growth increased significantly only after 730 days from the CMS addition. The highest dose of CMS caused that, at the end of the incubation period, pH value have increased 2 units, whereas bacterial growth was 4–10 times higher. In view of these results, CMS amendment could be considered as an agronomic sound practice for strongly acid soils (pH <4.5).