A new HPLC-MS/MS method for the simultaneous determination of 36 polyphenols in blueberry, strawberry and their commercial products and determination of antioxidant activity

 Food Chem. 367, 130743, 2022

Berry fruits consumption has increased in recent years because they are rich sources of polyphenols with reported health benefits. The aim of the present work was to develop a new comprehensive and fast HPLC-MS/MS method for simultaneous determination of 36 phenolic compounds (7 anthocyanins, 9 flavonols, 4 flavan-3-ols, 2 dihydrochalcones, 2 flavanones and 12 phenolic acids) present in blueberry, strawberry, and their fruit jam. Blueberry fruits showed higher contents of anthocyanins, flavonols and phenolic acids, while strawberry fruits exhibited higher contents of flavan-3-ols, dihydrochalcones and flavanones. Anthocyanins were the main phenolic constituents in both berries. Furthermore, the higher total phenolic content in the blueberry fruit and jam justified their greater antioxidant capacity measured by DPPH free radical assay, compared to strawberry. In conclusion, this new HPLC-MS/MS method is useful and reliable for quality control and authentication analyses of blueberry and strawberry fruits and their commercial food products, such as jams.

Avances en LC/MS/MS para dar Respuesta a los Desafíos en Acuicultura

Propuestas

·       Puesta en valor de una tecnología sólida y ampliamente probada.

·       Presentación de los últimos avances tecnológicos en acoplamientos LC/MS, en baja y alta resolución. 

·       Capacidad de análisis, tanto cualitativos como cuantitativos de gran calidad, simultáneamente. 

·       Solución total integrada, con el software completo de tratamiento de datos.

Agenda 

10:00-10:15. Recepción y entrega de documentación

10:15-10:30. Bienvenida y presentación

• Dr. Jesús Simal-Gándara,Director Dpto. Química Analítica y Alimentaria (UVigo)
• Dr. Javier Lago, SCIEX

10:30-11:00. Estrategias para la determinación y confirmación de biotoxinas

• Dra. Ana Gago-Martínez, Directora del Laboratorio de Referencia de la Unión Europea para Biotoxinas Marinas

11:00-11:30. Nuevas tecnologías: Rompiendo barreras y abriendo nuevos horizontes en TQ LC-MS/MS

• Dr. Josep Lluis Lliberia, SCIEX

11:30-12:00. Pausa / Café

12:00-12:30. Photodegradation study of different classes of organic contaminants in the aquatic environment: non-target by-product identification by on-line SPE-LC-(QTOF)-HRMS

• Dr. Thierry Dagnac, Agronomic and Agrarian Research Centre (INGACAL-CIAM)

12:30-13:00. Determinación de residuos en Acuicultura 

• Dr. Manuel Lolo, AMS LAB

13:00-13:30. Nuevas tecnologías: Rompiendo barreras y abriendo horizontes en HR-MS LC-MS/MS

• Dr. Josep Lluis Lliberia, SCIEX

13:30-14:00. Preguntas y comentarios

High-throughput HPLC–MS/MS determination of the persistence of neonicotinoid insecticide residues of regulatory interest in dietary bee pollen

Analytical and Bioanalytical Chemistry

DOI 10.1007/s00216-015-8870-4

The aim of this work is the development of a simple, fast, quantitative, and economic method for the determination of neonicotinoid insecticide residues in dietary bee pollen. Several parameters of the method, such as extraction solvent, extraction time, and solid-phase extraction sorbents for purification [silica, C18, primary–secondary amine (PSA), and Envi-Carb II/PSA], were studied. The final proposed method based on solid–liquid extraction with hexane, cleanup with Supelclean™ Envi-Carb II/PSA cartridges, and subsequent analysis by high-performance liquid chromatography with tandem mass spectrometry was validated and applied to the analysis of commercial bee pollen samples from different geographical zones. Method performance was assessed by the evaluation of several quality parameters of the method, such as recovery values, repeatability, reproducibility, linearity, and limits of detection and quantification. Matrix effects on the chromatographic signal were also studied. The quality parameters of the method were equivalent to or better than those obtained with previously published methods, with recoveries between 81 and 99 % and repeatabilities lower than 8.8 %. The detection and quantification limits were in the ranges 0.2-2.2 μg kg-1 and 0.4-4.3 μg kg-1, respectively.