Tuesday, 15 March 2016

Influence of pH on viscoelastic properties of heat-induced gels obtained with a β-Lactoglobulin fraction isolated from bovine milk whey hydrolysates

Food Chemistry, 219, 2017, 169–178


A β-Lactoglobulin fraction (r-βLg) was isolated from whey hydrolysates produced with cardosins from Cynara cardunculus. The impact of the hydrolysis process on the r-βLg structure and the rheological properties of heat-induced gels obtained thereafter were studied at different pH values. Differences were observed between r-βLg and commercial β-Lg used as control. Higher values for the fluorescence emission intensity and red shifts of the emission wavelength of r-βLg suggested changes in its tertiary structure and more solvent-exposed tryptophan residues. Circular dichroism spectra also supported these evidences indicating that hydrolysis yielded an intermediate (non-native) β-Lg state.

The thermal history of r-βLg through the new adopted conformation improved the microstructure of the gels at acidic pH. So, a new microstructure with better rheological characteristics (higher conformational flexibility and lower rigidity) and greater water holding ability was founded for r-βLg gel. These results were reflected in the microstructural analysis by scanning electron microscopy.

Monday, 14 March 2016

Contributions of a compost-biochar mixture to the metal sorption capacity of a mine tailing

Environmental Science and Pollution Research
February 2016, Volume 23, Issue 3, pp 2595–2602
DOI: 10.1007/s11356-015-5489-0


One technique applied to restore degraded or contaminated soils is to use amendments made of different types of waste materials, which in turn may contain metals such as Cu, Pb and Zn. For this reason, it is important to determine the capacity of the soil to retain these materials, and to compare the sorption capacity between an amended soil and another unamended soil. The aim of this study was to determine the mobility and availability of these metals in the soil after applying the amendment, and how it affected the soil’s sorption capacity. Sorption isotherms were compared with the empirical models of Langmuir and Freundlich to estimate the sorption capacity. The overall capacity of the soils to sorb Cu, Pb or Zn was evaluated as the slope Kr. The amendments used in this study were a mixture made of compost and biochar in different proportions (20, 40, 60, 100 %), which were applied to the mine tailing from a settling pond from a copper mine. The mine tailing that were amended with the mixture of compost and biochar had a higher sorption capacity than the mine tailing from the unamended pond, and their sorption isotherms had a greater affinity towards Cu, Pb and Zn than the mine tailing that was studied. Therefore, the results obtained show that adding a mixture of compost and biochar favours the retention of Cu, Pb and Zn in mine tailing.

Sunday, 13 March 2016

Assessing the influence of technosol and biochar amendments combined with Brassica juncea L. on the fractionation of Cu, Ni, Pb and Zn in a polluted mine soil

Journal of Soils and Sediments
February 2016, Volume 16, Issue 2, pp 339–348
DOI: 10.1007/s11368-015-1222-3



Soil metal pollution is a widespread problem around the world and remediation of these soils is difficult. The objective of this study was to investigate the effect of two different strategies on the chemical fractions of metals in a soil of a depleted copper mine: (1) amending with a mixture of a technosol made of wastes and biochar and (2) amending combined with planting vegetation (Brassica juncea).

A 3-month greenhouse experiment was carried out to evaluate the effects of organic amendments and vegetation on the metal fractionation of Cu, Ni, Pb and Zn in a mine soil of the depleted copper mine at Touro (Spain). We compared the influence of organic amendments alone (technosol + biochar) and combined with mustard plants (Brassica juncea L.).

The results showed that amending with a technosol made of wastes promoted plant growth (from 0.7 to 2.9 g of biomass produced) and reduced the CaCl2-extractable metal concentration in soil, reduced the mobility factor of Cu from 18.3 to 1.6, Ni from 47.5 to 2.3 and Pb from 17.9 to 2.1, and also reduced the concentration of metals in the mobile soil fractions. It was not possible to grow up Brassica juncea plants in the untreated settling pond soil due to the extremely degraded conditions of that soil. However, the application of the used technosol increased the Pb and Zn pseudototal concentrations in the amended soils.

We conclude that the combination of amending with wastes and planting B. juncea provides little additional benefit for remediating a metal-polluted soil compared with incorporation of wastes alone.

Thursday, 3 March 2016

Contribution of waste and biochar amendment to the sorption of metals in a copper mine tailing

CATENA
Volume 137, February 2016, Pages 120–125
http://dx.doi.org/10.1016/j.catena.2015.09.010


One technique applied to restore degraded or contaminated soils is to use amendments made of different types of waste materials, which in turn may contain metals such as Cu, Pb and Zn. For this reason it is important to determine the capacity of the soil to retain these materials, and to compare the sorption capacity between an amended soil and another unamended soil. The aim of this study was to determine the chemical behaviour of these metals in the soil after applying the amendment, and how it affected the soil's sorption capacity. Another aim was to study the contribution of contaminating elements from the amendment itself. The amendments used in this study were a mixture made of waste material (sewage sludges, sludges from an aluminium plant, ash, food industry wastes, and sands from a wastewater treatment plant) and biochar (biomass of Acacia dealbata) (97%:3%) in different soil/amendment proportions. The soil was from a mine tailing. The mine tailings were amended with the mixture of waste and biochar which had a higher sorption capacity than the soil from the pond. The samples with amendment had a greater affinity for Cu, Pb and Zn than the mine soil. The results obtained show that adding a mixture made of waste and biochar favours the retention of Cu, Pb and Zn in mine tailing from metal mines.

Wednesday, 2 March 2016

Histolocalization of aldehyde accumulation in citral-treated Arabidopsis thaliana roots

Allelopathy Journal 37:71-76 · 2016


Growth and development of Arabidopsis thaliana seedlings treated with the essential oil component citral have been severely affected. We analyzed whether citral can enter and accumulate into the roots and we found that it interferes with the plant metabolism accumulating mainly in the differentiation zone. Besides, the distribution of stained zones varied with the experimental time.

Tuesday, 1 March 2016

Nickel, Lead and Zinc Sorption in a Reclaimed Settling Pond Soil

Pedosphere
Volume 26, Issue 1, February 2016, Pages 39-48
doi:10.1016/S1002-0160(15)60020-0


The wastes used to amend soils sometimes have high concentrations of metals such as nickel (Ni), lead (Pb) and zinc (Zn). To determine the capacity of soils to retain these metals, the sorption capacities of different mine soils with and without reclamation treatments (tree vegetation and waste amendment) for Ni, Pb and Zn in individual and competitive situations were evaluated using the batch sorption technique. The untreated settling pond soil had low capacity for Ni, Pb and Zn retention. The site amended with wastes (sewage sludges and paper mill residues) increased the sorption capacity most, probably because of the higher concentrations of soil components with high retention capacity such as carbon and clay fraction. No significant competition was observed between metals in the competitive sorption experiment, indicating that the maximum of sorption was not achieved by adding 0.5 mmol L−1 of metal. We can conclude that, despite the possible additions of Ni, Pb and Zn from wastes to degraded soils, sewage sludges and paper mill residues have a high sorption capacity that would prevent the metals from being in a mobile form.

Monday, 22 February 2016

A widely used spectrophotometric assay to quantify olive oil biophenols according to the health claim (EU Reg. 432/2012)

European Journal of Lipid Science and Technology
DOI: 10.1002/ejlt.201500313


The purpose of this work was to find a simple, cheap, and suitable method, among the most widely employed, able to guarantee a proper determination and quantification of the phenolic content of extra virgin olive oils (EVOOs), in order to satisfy the requirements of the specific health claim (EU Reg. 432/2012). Total phenolic content by Folin–Ciocalteu (FC) was used and compared versus phenolic profile by HPLC-UV, considering this latter as the most sensitive and specific method for evaluating the phenolic content. Both protocols were performed before and after an acid hydrolysis of the polar phenolic fraction that involves a break of the bound forms of hydroxytyrosol (HTyr) and tyrosol (Tyr), with a simplification of the phenolic profile, and quantification of their total free forms. Results of the phenolic compounds of twelve EVOOs, determined by the different analytical approaches, were statistically compared by means of two-tailed paired t-tests: data obtained by the FC assay (expressed as HTyr) before and/or after acid hydrolysis were statistically comparable with results obtained by acid hydrolysis-HPLC (as sum of HTyr and Tyr).

Saturday, 20 February 2016

Dissipation of fungicides residues along winemaking, and their effects on fermentation and the volatile composition of wines

Journal of Agricultural and Food Chemistry


The effects of four fungicides commonly used for the control of fungal diseases in vines and grapes in the course of winemaking was tested. The concentration of fungicide residues was monitored throughout the process in order to establish their kinetics of dissipation. In all cases the percentages of dissipation were higher than 68% which shows the detoxificant effect of the winemaking process. On the other hand, the effect of the fungicide residues on the aroma composition of Tempranillo red wines was tested.

Wednesday, 17 February 2016

Cu retention in an acid soil amended with perlite winery waste

Environmental Science and Pollution Research (2016), 23, 3789–3798
DOI: 10.1007/s11356-015-5639-4


The effect of perlite waste from a winery on general soil characteristics and Cu adsorption was assessed. The studied soil was amended with different perlite waste concentrations corresponding to 10, 20, 40 and 80 Mg ha−1. General soil characteristics and Cu adsorption and desorption curves were determined after different incubation times (from 1 day to 8 months). The addition of perlite waste to the soil increased the amounts of organic matter as well as soil nutrients such as phosphorus and potassium, and these increments were stable with time. An increase in Cu adsorption capacity was also detected in the perlite waste-amended soils. The effect of perlite waste addition to the soil had special relevance on its Cu adsorption capacity at low coverage concentrations and on the energy of the soil-Cu bonds.

Tuesday, 16 February 2016

Xylitol production in immobilized cultures: a recent review

Critical Reviews in Biotechnology 36 (2016)


Xylitol is a pentahydroxy sugar alcohol coming from xylose with many applications in the food and pharmaceutical industries as a low caloric sweetener suitable for diabetics and as an active ingredient in several biomedical applications. The microbial bioproduction of xylitol from natural xylose coming from lignocellulosic materials appears a sustainable and a promising alternative to chemical synthesis, which works at stronger reaction conditions and generates undesirable co-products which must be removed. There are several reviews that study the metabolic pathways in wild and transformed xylitol producing yeasts and the culture conditions that enhance xylitol accumulation, which are mainly related to the need of microaerobiose for the best producing wild yeasts. Nevertheless, there are relatively few studies focusing on the engineering aspects related to scalable systems and bioreactors that could result in a final industrial stage. This review explores recent advances on xylitol production using immobilized systems, which have been proposed to facilitate the reuse of the biocatalyst for extended periods and the main types of bioreactors available assayed for this purpose.

Monday, 15 February 2016

Oxidation of edible animal fats. Comparison of the performance of different quantification methods and of a proposed new semi-objective colour scale-based method

Food Chemistry, 217 (2017) 743–749


The agreement among the results determined for the main parameters used in the evaluation of the fat auto-oxidation was investigated in animal fats (butter fat, subcutaneous pig back-fat and subcutaneous ham fat). Also, graduated colour scales representing the colour change during storage/ripening were developed for the three types of fat, and the values read in these scales were correlated with the values observed for the different parameters indicating fat oxidation.

In general good correlation among the values of the different parameters was observed (e.g. TBA value correlated with the peroxide value: r = 0.466 for butter and r = 0.898 for back-fat). A reasonable correlation was observed between the values read in the developed colour scales and the values for the other parameters determined (e.g. values of r = 0.320 and r = 0.793 with peroxide value for butter and back-fat, respectively, and of r = 0.767 and r = 0.498 with TBA value for back-fat and ham fat, respectively).

Tuesday, 2 February 2016

Phenolics production from alkaline hydrolysis of autohydrolysis liquors

CyTA - Journal of Food, 14 (2016) 255-265


The objective of this work was to study the release, identification and characterization of phenolic compounds by saponification of non-isothermal autohydrolysis liquors of corn (Zea mays) cobs and Eucalyptus globulus. Corn cobs and E. globulus are lignocellulosic materials with high contents in cellulose and hemicelluloses. Efficient use of these materials can be achieved using autohydrolysis as the first stage of a biorefinery, resulting in a solid phase rich in cellulose and lignin, and a liquid phase containing essentially hemicelluloses-derived compounds and phenolic compounds from the partial solubilization of lignin. This work studied the combination of alkaline hydrolysis and extraction with organic solvents to maximize the amounts of released phenolic compounds since these compounds are interesting for their properties and food applications.

Monday, 1 February 2016

Airborne pollen trends in the Iberian Peninsula

Science of the Total Environment
DOI: 10.1016/j.scitotenv.2016.01.069


Airborne pollen monitoring is an effective tool for studying the reproductive phenology of anemophilous plants, an important bioindicator of plant behavior. Recent decades have revealed a trend towards rising airborne pollen concentrations in Europe, attributing these trends to an increase in anthropogenic CO2 emissions and temperature. However, the lack of water availability in southern Europe may prompt a trend towards lower flowering intensity, especially in herbaceous plants. Here we show variations in flowering intensity by analyzing the Annual Pollen Index (API) of 12 anemophilous taxa across 12 locations in the Iberian Peninsula, over the last two decades, and detecting the influence of the North Atlantic Oscillation (NAO). Results revealed differences in the distribution and flowering intensity of anemophilous species. A negative correlation was observed between airborne pollen concentrations and winter averages of the NAO index. This study confirms that changes in rainfall in the Mediterranean region, attributed to climate change, have an important impact on the phenology of plants.

Saturday, 16 January 2016

Agronomic performance of maize populations divergently selected for diferulate cross-linkage

Journal of Agricultural Science
DOI: 10.1017/S0021859615001161


The direct response of a divergent selection programme for total cell wall ester-linked diferulate concentration in maize pith stalk tissues and its indirect effect on cell wall degradability and corn borer resistance have been previously evaluated. Since increased total diferulate concentration is expected to improve crop performance in response to corn borers, the objective of the present research was to evaluate the indirect response of the divergent selection for diferulates on agronomic traits under corn borer infestation. For this purpose, five maize populations with contrasting total diferulate concentrations were evaluated four environments for performance under protected and infested conditions. Measured traits were: days to anthesis, days to silking, plant height, stalk lodging, grain moisture at harvest and grain yield. High diferulate populations showed a significant reduction in anthesis (precocity), and were 11 cm taller than the starting population, while low diferulate populations were 9 cm shorter, and showed nearly 1 t/ha lower grain yield than the original and high diferulate populations. The analysis showed that cycles of selection were positively correlated with flowering, plant height and grain yield. The infestations with borers produced >1 t/ha of reduction in grain yield; although the higher diferulate populations showed a better performance under infestation than the low diferulate populations. This positive effect on the grain yield by increasing diferulate content can be considered an extra in order to breed for resistance to corn borers.

Friday, 15 January 2016

Oleaceae cross-reactions as potential pollinosis cause in urban areas

Science of the Total Environment
DOI: 10.1016/j.scitotenv.2015.10.107


It is worth noting the allergological problems induced by a not accurate design of the ornamental vegetation in the parks and streets of the cities. Usually, in the Oleaceae family, only the olive pollen is considered an important aeroallergen but other species of the family could be an important source of airborne pollen allergens. Pollen from Fraxinus, Olea and Ligustrum and its main aeroallergens were sampled in the atmosphere of an urban area in North-Western Spain during 2011. The allergen bioaerosol content was quantified by using specific 2-site ELISA and Ole e 1 antibodies. The Fra e 1 and Lig v 1 allergens were detected by means Ole e 1 antibodies. This fact demonstrates the cross-reactivity between the main allergens of Fraxinus, Olea and Ligustrum, plants widely species used as ornamental in the cities. Therefore, the urban allergenic people sensitized to Olea pollen could present allergenic reactions during the winter (due to ash pollen allergens), the spring (caused by olive pollen allergens) and the early summer (triggered by the privet flowering). As a consequence, sensitivity to the pollen of one species may favour development of sensitivity to all three species as consequence of the priming effect. The combination of pollen count and the allergen quantification must be assessed in the epidemiologic study of allergic respiratory diseases.

Wednesday, 13 January 2016

Acidification of mixtures of granite powder and compost for reuse in plant production

Compost Science & Utilization, 24 (2016)


We evaluated the utilization of granite powder—a residue of stone cutting and polishing resulting from its preparation for construction and ornamental use—in the preparation of nursery potting mixtures, within a general objective of waste recycling and reuse. Granite powder was blended with two composts of different origins and properties: one elaborated from the organic fraction of municipal solid waste (MSW), and another from pine bark. Since all materials presented pH values over 8, which are excessive for growing most vegetal species, the use of iron sulphate to acidify them has been assessed. Three doses of iron sulphate (14, 69, and 97 g kg−1, equivalent to 0.1, 0.5, and 0.7 meq H+ g−1) were added to the granitic powder and mixtures with 25 or 75% (v/v) of each compost, which were incubated in the laboratory during 30 days. Doses of iron sulphate above 0.1 meq H+ g−1 resulted in excessive electrical conductivity (>2 dS m−1) in all the samples, and too low pH values (<5) in most of them. The productivity of granite powder/compost mixtures acidified with 0.1 meq H+ g−1 was tested in a greenhouse experiment with ryegrass, where it was observed that plant productivity increased with respect to a control commercial substrate based on peat, in particular when the MSW compost was employed. On the basis of the physicochemical properties of the mixtures and the results of the greenhouse experiment, the use of granite powder mixed with 75% of MSW compost (v/v), acidified with 14 g kg−1 of iron sulphate (0.1 meq H+ g−1) is recommended.

Tuesday, 12 January 2016

Application of transit data analysis and artificial neural network in the prediction of discharge of Lor River, NW Spain

Water Science & Technology (2016)
DOI: 10.2166/wst.2016.002


Transit data analysis and artificial neural networks (ANNs) have proven to be a useful tool for characterizing and modelling non-linear hydrological processes. In this paper, these methods have been used to characterize and to predict the discharge of Lor River (North Western of Spain), one, two and three days ahead. Transit data analyses have showed a coefficient of correlation of 0.53 for a lag between precipitation and discharge of one day. By other hand, temperature and discharge has a coefficient of correlation negative (−0.43) for a delay of 19 day. The ANNs developed provide a good result for the validation period, with R2 between 0.92 and 0.80. Furthermore, these prediction models have been tested with discharge data from a period of 16 years later. Results of this testing period also show a good correlation with a R2 between 0.91 and 0.64. Overall, results indicate that ANNs are a good tool to predict river discharge with a small number of input variables.

Sunday, 10 January 2016

Variedades tolerantes a los taladros

 Agricultura: Revista agropecuaria, 992 (2016) 146-150


Saturday, 9 January 2016

Acidification of mixtures of granite powder and compost for reuse in plant production

COMPOST SCIENCE & UTILIZATION, 2016, 24, 1-10 



We evaluated the utilization of granite powder—a residue of stone cutting and polishing resulting from its preparation for construction and ornamental use—in the preparation of nursery potting mixtures, within a general objective of waste recycling and reuse. Granite powder was blended with two composts of different origins and properties: one elaborated from the organic fraction of municipal solid waste (MSW), and another from pine bark. Since all materials presented pH values over 8, which are excessive for growing most vegetal species, the use of iron sulphate to acidify them has been assessed. Three doses of iron sulphate (14, 69, and 97 g kg¡1, equivalent to 0.1, 0.5, and 0.7 meq HC g¡1) were added to the granitic powder and mixtures with 25 or 75% (v/v) of each compost, which were incubated in the laboratory during 30 days. Doses of iron sulphate above 0.1 meq HC g¡1 resulted in excessive electrical conductivity (>2 dS m¡1) in all the samples, and too low pH values (<5) in most of them. The productivity of granite powder/compost mixtures acidified with 0.1 meq HC g¡1 was tested in a greenhouse experiment with ryegrass, where it was observed that plant productivity increased with respect to a control commercial substrate based on peat, in particular when the MSW compost was employed. On the basis of the physicochemical properties of the mixtures and the results of the greenhouse experiment, the use of granite powder mixed with 75% of MSW compost (v/v), acidified with 14 g kg¡1 of iron sulphate (0.1 meq HC g¡1) is recommended.

Friday, 8 January 2016

Hydroxycinnamate Synthesis and Association with Mediterranean Corn Borer Resistance

J. Agric. Food Chem., 2016, 64, 539–551
DOI: 10.1021/acs.jafc.5b04862


Previous results suggest a relationship between maize hydroxycinnamate concentration in the pith tissues and resistance to stem tunneling by Mediterranean corn borer (MCB, Sesamia nonagrioides Lef.) larvae. This study performs a more precise experiment, mapping an F2 derived from the cross between two inbreds with contrasting levels for hydroxycinnamates EP125 × PB130. We aimed to co-localize genomic regions involved in hydroxycinnamate synthesis and resistance to MCB and to highlight the particular route for each hydroxycinnamate component in relation to the better known phenylpropanoid pathway. Seven quantitative trait loci (QTLs) for p-coumarate, two QTLs for ferulate, and seven QTLs for total diferulates explained 81.7, 26.9, and 57.8% of the genotypic variance, respectively. In relation to borer resistance, alleles for increased hydroxycinnamate content (affecting one or more hydroxycinnamate compounds) could be associated with favorable effects on stem resistance to MCB, particularly the putative role of p-coumarate in borer resistance.

Thursday, 7 January 2016

Biochemical, physiological and isotopic responses to natural product p-hydroxybenzoic acid in Cocksfoot (Dactylis glomerata L.)

Plant Growth Regulation
DOI: 10.1007/s10725-014-9981-1


The present study was conducted to evaluate the role of natural compound p-hydroxybenzoic acid (pHBA) on physiological, biochemical and isotopic responses of Dactylis glomerata L. Application of pHBA in the range 0.5–1.5 mM reduced the leaf fresh/dry biomass, shoot/root length, relative water content and leaf osmotic potential of D. glomerata. pHBA decreased the photosynthetic efficiency and quantum yield of photosystem II photochemistry in D. glomerata seedlings following treatment at all concentrations. Photochemical and non-photochemical fluorescence quenching were reduced after treatment with 1.5 mM pHBA. Carbon isotope composition ratio in D. glomerata leaves was significantly less negative following treatment with pHBA than the control. Carbon isotope discrimination value was declined by pHBA. The leaf protein content was lower after treatment with 1.5 mM pHBA. Our results suggest that pHBA possesses allelopathic potential against D. glomerata and this study provide new insights into the physiological, biochemical and isotopic action mechanism of pHBA.

Tuesday, 5 January 2016

The plant secondary metabolite citral alters water status and prevents seed formation in Arabidopsis thaliana

Plant Biology
DOI: 10.1111/plb.12418


Based on previous results, which showed that the secondary metabolite citral causes disturbances to plant water status, the present study is focused on demonstrating and detailing these effects on the water-related parameters of Arabidopsis thaliana adult plants, and their impact on plant fitness. Clear evidence of effects on water status and fitness were observed: plants treated with 1200 and 2400 μm citral showed decreased RWC, reduced Ψs, increased Ψw and reduced stomatal opening, even 7 days after the beginning of the experiment. Plant protection signals, such as leaf rolling or increased anthocyanin content, were also detected in these plants. In contrast, 14 days after beginning the treatment, treated plants showed signs of citral-related damage. Moreover, the reproductive success of treated plants was critically compromised, with prematurely withered flowers and no silique or seed development. This effect of citral on fitness of adult plants suggests a promising application of this natural compound in weed management by reducing the weed seed bank in the soil.

Thursday, 17 December 2015

Forecasting Olea Airborne Pollen Concentrations by Means of Artificial Intelligence

Fresenius Environmental Bulletin, (2015) 24, 4574-4580


A model based in an Artificial Neural Network was de- veloped in order to forecast the Olea airborne pollen concen- tration due to the allergenic power of its pollen grains. Daily data for Olea pollen and the meteorological variables docu- mented in the period 1993-2008, were used. All developed models had five input variables; i) Julian day, ii) precipita- tion, iii) humidity value, and iv) insolation hours. The model was carried out with data collected in the city of Ourense, North West of Spain. The developed model predicts the at- mospheric concentration of Olea pollen one day ahead. The model was tested with data from 2009 and 2010. The devel- oped model could be employed in allergology and health sci- ences in order to prevent effect of pollinosis. It is due the ability of obtain good predictions of threshold pollen con- centration values, which are important to take preventive measures by Health Systems. The time-lag observed in pre- diction phase may be due to the influence of other meteoro- logical parameters which have not been taken into account in the beginning, this fact is reaffirmed by studying the simi- larity between the different pollen seasons and the year 2010 (Mann-Whitney U Test with p<0.031), or due the low concen- trations of Olea pollen during the MPS (≈ 14 grains·m-3).

Thursday, 10 December 2015

Organic pollutants profiling of wood ashes from biomass power plants linked to the ash characteristics

Science of the Total Environment, 544 (2016) 535-543
doi:10.1016/j.scitotenv.2015.11.134



Wood ash, characterized by high content of certain nutrients and charcoal, can be applied to soils as a means of managing this waste product improving the soil quality. The associated environmental risk must be assessed. The objective of this study was to characterize the bottom and fly ash collected from 15 biomass power plants in Spain by determining the benzene, toluene, ethylbenzene, xylene and styrene (BTEX + S), PAHs and aliphatic hydrocarbon contents of both types of ash. Biochar was also used for comparison purposes.

Wednesday, 9 December 2015

As(V) and P Competitive Sorption on Soils, By-Products and Waste Materials

Int. J. Environ. Res. Public Health 2015, 12(12), 15706-15715
doi:10.3390/ijerph121215016


Batch-type experiments were used to study competitive As(V) and P sorption on various soils and sorbent materials. The materials assayed were a forest soil, a vineyard soil, pyritic material, granitic material, coarsely and finely ground mussel shell, calcinated mussel shell ash, pine sawdust and slate processing fines. Competition between As(V) and P was pronounced in the case of both soils, granitic material, slate fines, both shells and pine sawdust, showing more affinity for P. Contrary, the pyritic material and mussel shell ash showed high and similar affinity for As(V) and P. These results could be useful to make a correct use of the soils and materials assayed when focusing on As and P removal in solid or liquid media, in circumstances where both pollutants may compete for sorption sites.

Monday, 7 December 2015

Electrical Percolation of AOT-based Microemulsions with n-Alcohols

Journal of Molecular Liquids
DOI: 10.1016/j.molliq.2015.12.021


Percolative behaviour of w/o AOT/iC8/H2O microemulsions added with different n-alkanols is reported. 1-n-alcohols and 2-n-alkanols presented dissimilarities affecting percolation. Smaller alcohols ease percolation, especially at low concentrations. Greater molecules implied a reinforcement of the surfactant film that delayed percolation threshold. Also, a neural network based simulation model of the phenomenon has been developed. This single model has only five input variables and can estimate percolation temperature of microemulsions added with the two types of alcohols studied, with an RMSE of 0.98oC and R2=0.9740 (validation dataset values). This is considered a successful prediction rate, following previous developments with other families of additives, that confirms neural networks as reliable tools for percolative behaviour modelling.

Thursday, 3 December 2015

A palla de cereal reinvéntase como material de última xeración para producir nanofibras de celulosa


  • Investigadores das universidades de Córdoba e Vigo desenvolven novos usos para estes residuos a través de novas novas técnicas de separación ambientalmente favorables.


A industria agroalimentaria enfróntase ao reto de xestionar os seus residuos e faino, entre outras liñas de actuación, buscando a través da I+D novos usos para os subprodutos que xera. É o caso da palla de cereal. Os cambios nos usos dos terreos agrícolas, máis orientados actualmente á obtención de alimentos e de biocombustible, traen consigo a necesidade e a oportunidade de aproveitar os residuos de cultivos de cereais como o trigo, a cebada ou a avea. Investigadores da Universidade de Vigo colaboran nun proxecto da Universidade de Córdoba que traballa na obtención de nanofibras de celulosa, actualmente obtidas principalmente de especies madereiras, destes residuos agrícolas. 

Os resultados desta investigación conxunta veñen de ser publicados na revista Bioresource Technology, nun artigo titulado Agricultural residue valorization using a hydrothermal process for second generation bioethanol and oligosaccharides production e asinado por F. Vargas, E. Domínguez, C. Vila, A. Rodríguez Pascual e Gil Garrote, investigador este último da Facultade de Ciencias do campus de Ourense. Segundo explican os científicos do equipo de investigación Biorrefinería de Materiais Lignocelulósicos da Industria Agroalimentaria da Universidade de Córdoba, liderado por Alejandro Rodríguez, a celulosa é un dos polímeros máis dispoñibles do planeta pola súa presenza nos vexetais. Este grupo ten unha ampla bagaxe na obtención de papel e cartón a partir da celulosa, buscando agora novos procedementos químicos para obter máis produtos e de maior valor engadido, como hidroxeles ou compoñentes para a formulación de cemento.


Obtención de azucres e biocombustible

Entre estes procedementos está a biorrefinería de materiais lignocelulosos, que permite separar nos residuos agrícolas como a palla de cereal a celulosa, a hemicelulosa e a lignina. O primeiro material é aproveitable directamente para facer papel desde a celulosa chamada nobre, mentres que coas hemicelulosas obtéñense compostos de interese industrial, como por exemplo xilitol. Por último, está a lignina, que está presente na chamada lixivia negra ou licor residual. A colaboración coa Universidade de Vigo permitiu desenvolver novas técnicas na separación da celulosa e a lignina. O proceso, de carácter innovador, disolve a materia prima cun catalizador ácido, obtendo unha fracción rica en hemicelulosas e outra rica en celulosa e lignina. A partir desta fracción líquida caracterizáronse azucres interesantes para a industria alimentaria, como xilitol. A fracción sólida, rica en celulosa, púidose fermentar para a obtención de biocombustibles. 

Segundo comenta Rodríguez Pascual, “a palla do cereal ata o de agora empregábase para alimentar o gando ou se desbotaba no campo, en ocasións por medio de pequenos lumes controlados”. O procedemento desenvolvido durante a investigación parte dun proceso ambientalmente favorable, ao empregar sosa a presión atmosférica. Así obtéñense fibras celulósicas por un lado, que se utilizarán para a obtención de lignonanofibras de celulosa (con aparencia de xel), e o licor negro, rico en lignina, por outro. Os investigadores están obtendo lignonanofibras de celulosa mediante procedementos mecánicos, enzimáticos e por oxidación TEMPO, partindo da pasta celulósica sen branquexar, o que implica un menor custe económico e ambiental respecto a outras nanofibras que se obteñen mediante un proceso previo de blanqueamiento.

Fuente: DUVI

Wednesday, 25 November 2015

Influence Prediction of Alkylamines Upon Electrical Percolation of AOT-based Microemulsions Using Artificial Neural Networks

Tenside Surfactants Detergents, 52, 473-476 (2015)


Simulations for the electrical percolation of AOT/iC8/H2O w/o microemulsions added with alkylamines have been carried out by means of multilayer perceptron. Five variables have been elected as inputs: amine concentration, molecular weight, log P, hydrocarbon chain length (as number of carbons), and pKa. As a result, a neural model consisting in five input neurons, two middle layers (with fifteen and ten neurons respectively) and one output neuron was chosen because of its better performance, with a RMSE of 0.54 °C for the prediction set, with R2 = 0.9976.




Sunday, 22 November 2015

Metales pesados en suelos de pradera de A Pastoriza (NO España) tratados con purín de vacuno y fertilizantes NPK

Spanish Journal of Soil Science, 5 (2015) 154-164


En Galicia las praderas ocupan una gran extensión, siendo utilizadas principalmente para la alimentación del ganado vacuno destinado tanto a la producción de leche como de carne. Las granjas se manejan de manera intensiva, utilizando fertilizantes inorgánicos y purín de vacuno para aumentar la producción de pastos. Esta práctica puede aumentar el contenido de metales pesados en el suelo. En este trabajo se estudian las concentraciones totales y la distribución en profundidad de metales pesados y elementos afines (As, Cd, Cr, Cu, Mn, Ni, Pb y Zn) en dos suelos forestales (SN1, SN2) y en cinco praderas (P1-P5) fertilizadas con purín de vacuno y NPK en una zona con una gran vocación ganadera (A Pastoriza, Lugo). Se pretende conocer el grado de influencia de la fertilización sobre los niveles de estos elementos. Los suelos elegidos se desarrollaron a partir de pizarras (SN2, P4) y de cuarcitas de la Serie de Cándana (SN1, P1, P2, P3, P5). Los suelos forestales presentaron pH ácido (4,58-4,68), elevada saturación del complejo de cambio por Al (75-90%) y baja concentración de P disponible (4,78-11,96 mg kg-1); estos parámetros mejoran en los suelos de pradera como consecuencia de las enmiendas y fertilizantes aplicados, presentando un pH entre 5,17 y 7,02, una saturación de Al que varía entre 0,58-59,24% y P disponible entre 5,24 y 42,07 mg kg-1.En relación con los metales pesados, la profundidad de la muestra no afecta de modo significativo a su concentración total, pero sí el material de partida, presentando concentraciones más elevadas de Fe, As, Cu y Ni los suelos desarrollados sobre pizarras, posiblemente debido a la presencia de materiales piríticos. En la mayoría de los casos, los metales pesados estudiados presentan concentraciones totales inferiores a las establecidas como genéricas para suelos desarrollados sobre los respectivos materiales geológicos, y siempre son menores que los límites considerados fitotóxicos. La fertilización aplicada no parece tener una influencia significativa sobre los contenidos totales de estos elementos, con la excepción del Zn en la pradera P4, ya que los suelos naturales en muchos casos presentan valores similares e incluso superiores a los obtenidos en las parcelas fertilizadas.

Wednesday, 18 November 2015

Dissipation kinetics of pre-plant pesticides in greenhouse-devoted soils

Science of the Total Environment, 543 (A) (2016) 1-8


This work was conducted to study the distribution of methyl isothiocyanate (MITC) in greenhouse soils treated with the fumigant dazomet (DZ) from the formulated product “Basamid Granular®”, but also of 1,3-dichloropropene (1,3-D) and chloropicrin (CP) from the fumigant “Agrocelhone NE®”. In order to achieve this aim, several methods for the determination of fumigants residues in soils, but also pepper fruits were optimized and characterized. With independence of the soil depth, no residues of MITC, 1,3-D and CP above the detection limits were observed in soils covered with a polyethylene (PE) film (0.04 mm thick) after 27, 13 and 8 days of treatment, respectively. Liberation and dissipation curves of MITC in soil in presence of a PE film (0.04 mm) used to limit volatilization losses were also obtained. According to the results, the rate of decomposition of DZ into MITC have a half-life of 3.7 days in the surface horizon (5–10 cm) of the soil while in the subsurface horizon (15–20 cm), MITC formation rate is slightly slow (half-life of 3.2 days). With respect to the dissipation process, half-lives lower than 1 day were obtained for both depths (0.8 and 0.9 for the surface and the subsurface horizon, respectively). In the case of 1,3-D and CP in soil, the dissipation half-life of 1,3-D on soils was a bit higher than for CP (2 days vs. 1). In addition, the presence of residues of the fumigants on green pepper fruits grown on the treated soils was not detected as expected.


Sunday, 15 November 2015

Voltammetric analysis of mancozeb and its degradation product ethylenethiourea

Journal of Electroanalytical Chemistry, 758 (2015) 54-58


The purpose of this work was to develop a reliable alternative method for the determination of the dithiocarbamate pesticide mancozeb (MCZ) in formulations. Furthermore, a method for the analysis of MCZ's major degradation product, ethylenethiourea (ETU), was also proposed. Cyclic voltammetry was used to characterize the electrochemical behavior of MCZ and ETU, and square-wave adsorptive stripping voltammetry (SWAdSV) was employed for MCZ quantification in commercial formulations.

It was found that both MCZ and ETU are irreversibly reduced (− 0.6 V and − 0.5 V vs Ag/AgCl, respectively) at the surface of a glassy carbon electrode in a mainly diffusion-controlled process, presenting maximum peak current intensities at pH 7.0 (in phosphate buffered saline electrolyte).

Several parameters of the SWAdSV technique were optimized and linear relationships between concentration and peak current intensity were established between 10–90 μmol L− 1 and 10–110 μmol L− 1 for MCZ and ETU, respectively. The limits of detection were 7.0 μmol L− 1 for MCZ and 7.8 μmol L− 1 for ETU. The optimized method for MCZ was successfully applied to the quantification of this pesticide in two commercial formulations. The developed procedures provided accurate and precise results and could be interesting alternatives to the established methods for quality control of the studied products, as well as for analysis of MCZ and ETU in environmental samples.

Friday, 6 November 2015

“Nun churrasco, hai máis risco de cancro co sol que coa carne”


Fonte: GCiencia

A decisión da OMS de declarar canceríxena a carne procesada debe entenderse en relación á cantidade e o tempo, segundo Jesús Simal, catedrático da Universidade de Vigo.

Desterrar o touciño das nosas comidas? Renunciar ao xamón ou non comelo tanto como nos gustaría? O churrasco, perigoso? Poucas veces unha declaración da Organización Mundial da Saúde (OMS), apoiada polos científicos da Axencia Internacional para a Investigación en Cancro (IARC), é capaz de xerar tanta alarma e discordia. A carne procesada – sometida a procesos de salgadura, como o lacón, ou curada, como o tótem da gastronomía ibérica que é o xamón, pero tamén hamburguesas ou salchichas- entran no grupo 1 de alimentos carcinóxenos. A carne vermella queda baixo sospeita, nun grupo 2A que a sitúa como “probablemente” carcinóxena. Que o tabaco e o lacón estean segundo a OMS no mesmo grupo de produtos canceríxenos fai  tentadora a comparación entre o cigarro e os banquetes carnívoros, máis os científicos que traballan para botar luz sobre a relación entre o consumo de carne e o cancro colorrectal relaxan a alarma. Tamén a propia OMS o fai: é cuestión de cantidades e de tempo.

Jesús Simal é catedrático da área de Coñecemento de Nutrición dirixe o Departamento de Química Analítica e Alimentaria da Universidade de Vigo, no campus de Ourense. As súas investigacións máis recentes versan sobre o factor cárnico no risco de padecer un cancro. Ante a alarma, lembra que é importante ter en conta o tipo de consumo. “O que vén dicir a Axencia Internacional para a Investigación en Cancro”,  interpreta o investigador, “é que altos consumos diarios de carnes procesadas, especialmente as tratadas a altas temperaturas, con procesos de fritura ou á grella, contribúen a un maior risco de cancro colorrectal”. Con todo, o científico lembra que para avaliar o risco real convén agardar o informe da Autoridade Europea de Seguridade Alimentaria. “Os seus ditames serven para analizar a relación entre risco e beneficio derivado do consumo dos alimentos, para así emitir recomendacións en función desta avaliación dunha maneira prudente”, asegura. Ademais, hai detalles sobre o traballo que só se coñecerán cando o IARC publique o estudo monográfico completo nuns meses.

Ao alarmismo xerado pola decisión da OMS Simal recéitalle decisións informadas. “Hai que ter en conta que o risco cero non existe; trátase de minimizalo no posible. Vivir significa precisamente tomar decisións poñendo na balanza vantaxes e inconvenientes. O que si é adecuado é estar informado sobre a magnitude do perigo para calibrar o risco asociado. Son moitos os factores de risco, así que non ten moito sentido atribuílo a un único factor”, opina Simal. Así, na análise dos perigos hai tamén algún paradoxo. “Por exemplo”,  segue o investigador, “no consumo de carne á grella o maior risco de cancro estaría en non usar protector solar mentres se prepara ou mesmo na inhalación de partículas finas de carbón transportados polo fume”.

O cancro é unha sorte de tómbola na que se entra a xogar con máis ou menos papeletas. “Por razóns de tipo xenético, unhas persoas están máis predispostas que outras a padecer un ou outro tipo de enfermidades. Ademais, os nosos hábitos, como a dieta, o exercicio físico, o fumar, o beber alcohol ou a exposición a contaminantes poden condicionar a expresión ou non de xenes que desencadean este tipo de patoloxías. É por iso que é moi importante un equilibrio na dieta de acordo coa pirámide de alimentos saudables: moderar as carnes vermellas procesadas a altas temperaturas e soster un consumo regular dos alimentos recomendados a diario, na súa maioría produtos hortofrutícolas con ingredientes antioxidantes, que reducen o dano oxidativo a nivel celular”. As recomendacións existentes sobre hábitos de vida saudables xa situaban a carne vermella e os seus subprodutos no cumio da pirámide, a dos alimentos ocasionais.

Simal estudou en 2014 xunto a un equipo das universidades de Vigo e Porto os compoñentes canceríxenos da carne á grella e como reducir o seu impacto ao cociñalos. Para entender o aviso da OMS hai que ter en conta que ao asar carne de porco, por exemplo, se están formando uns compoñentes canceríxenos, os hidrocarburos aromáticos policíclicos. “Fórmanse ao contacto directo da carne coa chama, ou con superficies metálicas quentes, pero tamén ao gotear os mollos cárnicos, graxas sobre todo, sobre as brasas. O fume con estes compostos deposítase sobre a superficie da carne, de aí o interese en reducir e limitar estes compostos a doses seguras. Nós estudamos se o marinado previo con cervexas de distinta actividade antioxidante podía reducir os niveis destes compostos. Atopamos que a cervexa negra, de maior actividade antioxidante, reducía os seus niveis nun 60%. A capacidade de inhibición da súa formación aumentou coa capacidade antioxidante da cervexa, o que abre a porta ao estudo do marinado con produtos ricos en antioxidantes, como o viño tinto ou o té, ademais de allos e cebolas unidos a adobos de vinagre ou limón. Contribúen a cambiar a acidez na superficie da carne e preveñen que se fixen a ela os compostos canceríxenos do fume”,explica o catedrático da Universidade de Vigo.

O equilibrio na dieta e manter algúns bos hábitos á hora de cociñar parecen mellor resposta que a de declararlle guerra aberta á churrascada ou ao xamón. Simal recomenda, por exemplo, ter ben limpas as grellas antes de usalas para evitar o contacto da carne con restos xa carbonizados, ou envolver as pezas de carne en papel aluminio para que non haxa contacto directo coa chama ou con superficies metálicas quentes.  Engadir á carne asada algunha hortaliza aporta antioxidantes ao prato.

Algúns trucos e sentido común na dieta, pero tamén a ciencia debería poder reducir os compoñentes canceríxenos das carnes procesadas, lembra Simal. “Os embutidos e o lacón, así como o outros produtos crus curados a base de carnes vermellas obtidas por salgadura, curado, fermentación e afumado, conteñen na súa composición nitratos e nitritos como conservantes para evitar a proliferación de microorganismos patóxenos ou manter a cor vermella das carnes. Poden formar derivados nitrosados como nitrosaminas e nitrosamidas canceríxenas. Por iso se limitan as doses residuais destes compostos a niveis seguros para os consumidores. Ademais estase investigando a substitución parcial ou total destes aditivos con extractos de plantas con propiedades antimicrobianas e antioxidantes. Malia este coñecemento, aínda non se comprende completamente como se incrementa o risco de cancro pola carne vermella ou procesada”.

O ditame da OMS alarma a sociedades consumidoras de carne e confirma o que o coñecemento científico – “sempre suxeito a revisión, estudo e modificación”, matiza Simal- xa advertía: que un consumo excesivo de carne –máis de 500 gramos á semana xa  é demasiado-  aumenta o risco dun cancro colorrectal. Pero dentro dunha dieta equilibrada non hai razóns para desterrar as carnes nin os seus nutrintes. “O problema é que cada vez somos máis carnívoros, e consómese carne case a diario, máis en homes que en mulleres, e esta tendencia é peor nas xeracións máis novas. Ademais, a nosa maior lonxevidade fai que aumente o risco de enfermidades crónicas e dexenerativas, e de aí a conveniencia duns hábitos de vida saudables”.

A súa presenza varía en distintas rexións do mundo porque no que respecta á comida hai hábitos para describir até cansar. “Non existen patróns nutricionais bioloxicamente determinados, senón hábitos alimentarios culturalmente herdados”, lembra Simal, que lembra que o omnívoro ser humano pode apreciar “dende a termita até a balea e hai pobos como os inuit, que consomen sobre todo carne e peixe e outros que se alimentan case unicamente de cereais”.

Friday, 23 October 2015

O aceite galego, saudable e con valor diferencial


A produción de aceite en Galicia como actividade xeralizada é historicamente constatable ata o século XVIII. A partir dese momento comeza o seu declive ata case a súa desaparición. Foi a partir de ben entrada a década dos 90 cando se comezou a producir un tímido auxe da actividade oliveira na comunidade. Na actualidade por unha banda estase a fomentar a plantación de variedades principais, a arbequina e/ou picual, coa finalidade de alcanzar unha produción oleícola intensiva a curto prazo, e, doutra banda, estase a recuperar oliveiras centenarias das variedades coñecidas polos olivicultores locais como brava e mansa para elaborar aceites únicos. Para protexer e garantir este valor diferencial dos aceites elaborados con exemplares de oliveiras centenarias, a investigadora Patricia Reboredo abordou na súa tese de doutoramento unha profunda análise das súas características.

A tese, titulada Caracterización aromática e fenólica de olivas e aceites de oliva producidos en Galicia, foi realizada na Facultade de Ciencias do campus de Ourense baixo a dirección de Jesús Simal, Beatriz Cancho e Carmen González, da Área de Nutrición e Bromatoloxía do Departamento de Química Analítica e Alimentaria da Universidade de Vigo. “En primeiro lugar, o noso obxectivo foi identificar correctamente este material vexetal mediante técnicas moleculares para demostrar que non se trata de variedades de oliveiras rexistradas no Banco de Germoplasma Mundial da Oliveira (BGMO) da Universidade de Córdoba”. Como resultado, detectouse que o perfil molecular das 20 mostras avaliadas coincidiu co da variedade brava, actualmente en fase de rexistro. Unicamente o perfil de dúas mostras non puido asociarse a ningunha das variedades incluídas en devandito BGMO. "Este feito pode deberse a que sexan variedades autóctonas descoñecidas ata o momento ou ben variedades sen introducir aínda no BGMO", comenta a investigadora.

Características aromáticas
Centrándose na análise das características dos aceites galegos, Reboredo sinala que “a calidade sensorial dos aceites elaborados con variedades principais está amplamente avaliada e recoñecida. Con todo, os aceites de oliva virxe obtidos a partir de variedades non clasificadas ata o momento, como os galegos, poderían presentar características diferentes e mesmo superiores a estes”. Se esto se demostra, engade, non só se podería potenciar a elaboración de aceites monovarietais destas variedades minoritarias senón que ademais se poderían deseñar novos aceites resultantes de procesos de moenda ou mestura con outras variedades. 

Na avaliación sensorial realizada durante a investigación, feita unicamente nos aceites elaborados con olivas brava e mansa en diferentes proporcións, os atributos "amargo" e "picante" destacan sobre todo no aceite elaborado cunha maior proporción de brava, mentres que os atributos "doce", "afroitado maduro" e "folla verde" son moi similares en ambos aceites. No desenvolvemento destes novos aceites, a comoenda ou mestura de olivas brava/mansa con arbequina ou picual aumentaron significativamente as series odorantes principais que conforman o perfil sensorial dos aceites e, ao mesmo tempo, a súa funcionalidade debido ao incremento dos compostos fenólicos. 

Na categoría de saudable
En relación á funcionalidade dos aceites, Patricia Reboredo recalca que a Comisión Europea aprobou recentemente a alegación de propiedade saudable para aqueles aceites que conteñan polo menos cinco mg de hidroxitirosol e os seus derivados/por 20 gr de aceite de oliva virxe, tendo en conta que os polifenoles do aceite de oliva contribúen á protección dos lípidos fronte ao dano oxidativo. Segundo apunta a xa doutora ourensá, “os resultados obtidos a partir dun protocolo analítico sinxelo e validado demostraron que os aceites elaborados a partir das olivas brava/mansa satisfán os requisitos esixidos no marco da Unión Europea para poder indicar na etiquetaxe a declaración de propiedade saudable, superando incluso o limiar establecido na lexislación”. 

Perfil aromático non extrapolable da oliva ao aceite
Durante a tese, Reboredo Rodríguez tamén analizou se era posible extrapolar este perfil aromático único das olivas galegas ao aceite. “O aceite de oliva virxe caracterízase, entre outros atributos, polo seu aroma asociado á presenza minoritaria de compostos volátiles que se transfiren ao aceite, en gran medida, desde a moenda do froito. Comprobar se o perfil aromático das olivas se pode extrapolar ao seu correspondente aceite permitiría avaliar o potencial aromático de cada variedade previamente á elaboración do aceite”, comenta a investigadora. 

En base aos resultados obtidos, demostrouse que o perfil aromático das olivas non é extrapolable posto que a actividade das encimas endóxenas ao longo do proceso de elaboración é o principal factor que determina a síntese dos compostos volátiles máis relevantes do aroma do aceite. Na investigación, demostrou que a actividade destas encimas pódese modular variando distintos parámetros durante o proceso de elaboración, sendo a temperatura e o tempo da etapa de batido os máis importantes. “A elección da combinación temperatura/tempo de batido dependerá de se se prioriza a súa funcionalidade ou o seu aroma, ademais de estar supeditada ao rendemento da extracción”, afirma por último.

Fonte: DUvi

Thursday, 22 October 2015

Postdoctoral position - Design of new polyurethane-based hydrogels


Topic:
Hydrogels find numerous applications in medicine. The goal of this project is to develop together with an industrial partner, new functional pre-polymers systems as a component of polyurethane based hydrogels. The project will be constituted of the synthesis and characterization of pre-polymers, the synthesis and preparation of hydrogels based on these polymers and finally by evaluation of physico-chemical, physical and mechanical properties of the resulting materials (swelling ratio, wettability, rheology, flexibility, mechanical properties, roughness of the surface, etc…)

The laboratory and the team:
This postdoctoral position will be carried out in the frame of an industrial cooperation at the laboratory of Photochemistry and Macromolecular Engineering (LPIM) in Mulhouse. The project will be held within the team Chemistry and Physico-Chemistry of Polymers under the supervision of Professors Sophie BISTAC and Jean-François STUMBE 

Profile of the applicant:
The applicant must have strong competencies in the area of synthesis and characterisation of polymers as well as in physico-chemistry of polymeric material. An experience in the specific area of complex macromolecular architectures and / or of polyurethanes (chemistry and physico-chemistry) is a must. In addition, experience in the domain of hydrogels will be strongly appreciated. Finally, the applicant should have a strong ability in French and/ or in English (spoken and written). 

For more information and for application, please contact:

Pr. Jean-François STUMBE 
Tel. ++33 (0)3 89 33 67 59

A detailed CV, plus motivation letter and reference letter(s) from the PhD supervisor and last(s) postdoc(s) supervisor are required for evaluation of the application.

Université de  Haute-Alsace
Laboratoire de Photochimie et d’Ingénierie Macromoléculaires
3 rue Alfred Werner - 68093 MULHOUSE CEDEX - FRANCE
Tél. +33 (0)389 335 025 - Fax +33 (0)389 335 014


Wednesday, 21 October 2015

A Critical Review on the Applications of Artificial Neural Networks in Winemaking Technology

Critical Reviews in Food Science and Nutrition


Since their development in 1943, artificial neural networks were extended into applications in many fields. Last twenty years have brought their introduction into winery, where they were applied following four basic purposes: authenticity assurance systems, electronic sensory devices, production optimization methods, and artificial vision in image treatment tools, with successful and promising results. This work reviews the most significant approaches for neural networks in winemaking technologies with the aim of producing a clear and useful review document.



Friday, 16 October 2015

Characterization of Xanthophyll Pigments, Photosynthetic Performance, Photon Energy Dissipation, Reactive Oxygen Species Generation and Carbon Isotope Discrimination during Artemisinin-Induced Stress in Arabidopsis thaliana

PLOS ONE
DOI:10.1371/journal.pone.0114826


Artemisinin, a potent antimalarial drug, is phytotoxic to many crops and weeds. The effects of artemisinin on stress markers, including fluorescence parameters, photosystem II photochemistry, photon energy dissipation, lipid peroxidation, reactive oxygen species generation and carbon isotope discrimination in Arabidopsis thaliana were studied. Arabidopsis ecotype Columbia (Col-0) seedlings were grown in perlite and watered with 50%Hoagland nutrient solution. Adult plants of Arabidopsis were treated with artemisinin at 0, 40, 80, 160 μM for one week. Artemisinin, in the range 40–160 μM, decreased the fresh biomass, chl a, b and leafmineral contents. Photosynthetic efficiency, yield and electron transport rate in Arabidopsis were also reduced following exposure to 80 and 160 μM artemisinin. The ΦNPQ and NPQ were less than control. Artemisinin treatment caused an increase in root oxidizability and lipid peroxidation (MDA contents) of Arabidopsis. Calcium and nitrogen contents decreased after 80 and 160 μM artemisinin treatment compared to control. δ13C values were less negative following treatment with artemisinin as compared to the control. Artemisinin also decreased leaf protein contents in Arabidopsis. Taken together, these data suggest that artemisinin inhibits many physiological and biochemical processes in Arabidopsis.

Thursday, 15 October 2015

Identification of Relevant Phytochemical Constituents for Characterization and Authentication of Tomatoes by General Linear Model Linked to Automatic Interaction Detection (GLM-AID) and Artificial Neural Network Models (ANNs)

PLOS ONE
DOI: 10.1371/journal.pone.0128566


There are a large number of tomato cultivars with a wide range of morphological, chemical, nutritional and sensorial characteristics. Many factors are known to affect the nutrient content of tomato cultivars. A complete understanding of the effect of these factors would require an exhaustive experimental design, multidisciplinary scientific approach and a suitable statistical method. Some multivariate analytical techniques such as Principal Component Analysis (PCA) or Factor Analysis (FA) have been widely applied in order to search for patterns in the behaviour and reduce the dimensionality of a data set by a new set of uncorrelated latent variables. However, in some cases it is not useful to replace the original variables with these latent variables. In this study, Automatic Interaction Detection (AID) algorithm and Artificial Neural Network (ANN) models were applied as alternative to the PCA, AF and other multivariate analytical techniques in order to identify the relevant phytochemical constituents for characterization and authentication of tomatoes. To prove the feasibility of AID algorithm and ANN models to achieve the purpose of this study, both methods were applied on a data set with twenty five chemical parameters analysed on 167 tomato samples from Tenerife (Spain). Each tomato sample was defined by three factors: cultivar, agricultural practice and harvest date. General Linear Model linked to AID (GLM-AID) tree-structured was organized into 3 levels according to the number of factors. p-Coumaric acid was the compound the allowed to distinguish the tomato samples according to the day of harvest. More than one chemical parameter was necessary to distinguish among different agricultural practices and among the tomato cultivars. Several ANN models, with 25 and 10 input variables, for the prediction of cultivar, agricultural practice and harvest date, were developed. Finally, the models with 10 input variables were chosen with fit’s goodness between 44 and 100%. The lowest fits were for the cultivar classification, this low percentage suggests that other kind of chemical parameter should be used to identify tomato cultivars.

Wednesday, 14 October 2015

El campus crea nuevos productos para el consumo humano y animal


El campus crea nuevos productos para el consumo humano y animal


En el apartado de calidad y seguridad alimentaria el CIA desarrolló en el último lustro cinco proyectos publicó más de 35 artículos en revistas de prestigio internacional. Uno de sus estudios actuales es comparar la riqueza en compuestos polifenólicos antioxidantes y compuestos volátiles aromáticos de productos hortofrutícolas nativos y de las variedades comerciales. La uva es protagonista de una parte de ese trabajo, que también estudia el efecto en el contenido y actividad aromática y funcional del producto de los tratamientos de conservación tras la cosecha y de los procesos de transformación de la industria. En el ámbito de la producción sostenible, este centro estudia los efectos de la actividad humana sobre el entorno natural en el que desarrolla la actividad agraria. Uno de sus proyectos es la interacción de los contaminantes agrarios y ambientales (plaguicidas, por ejemplo) con el sistema suelo-agua-cultivo-alimento. Los investigadores evalúan también la persistencia de estos residuos en los productos que consumimos. Doce proyectos y más de 50 artículos son el fruto directo del trabajo en los últimos 5 años en ese terreno. El tercer eje estratégico es el desarrollo de nuevos productos para la alimentación humana y animal, por ejemplo, presentaciones tipo surimi a partir de especies de pescado de baja demanda o recuperación de componentes bioactivos usando subproductos como sueros de leche.