Monday, 31 July 2017

Combination of food wastes for an efficient production of nisin in realkalized fed-batch cultures

Biochemical Engineering Journal


Nisin production by Lactococcus lactis CECT 539 was studied in four realkalized fed-batch cultures in diluted whey with feeding with lactose- and glucose-containing substrates. The first and third cultures were fed with mixtures of whey (W) and a 400 g/L concentrated glucose (CG), or with a concentrated mussel processing waste (CMPW) and CG, respectively.
The second and fourth cultures were respectively performed under the same conditions as in the first and third fermentations. However, these cultures were supplemented with mixtures of W plus a 2% (w/v) yeast extract (WYE2) and CG (second culture), or with CMPW plus a 2% (w/v) yeast extract (CMPWYE2) (fourth culture) after sample extractions at 132 and 168 h, respectively. From these times, each culture was fed with mixtures of WYE2 and CG, or CMPWYE2 and CG, respectively.
The final concentrations of nisin obtained in the third (223.98 BU/mL) and fourth (350.61 BU/mL) cultures, fed with glucose-containing substrates (CG and CMPW), were considerably higher than those obtained in the first (108.00 BU/mL) and second (158.53 BU/mL) cultures fed with the mixture of lactose- and glucose-containing substrates (W and CG).

Sunday, 30 July 2017

High hydrostatic pressure as pretreatment and adjuvant for the enzymatic release of ferulic acid from corn cob

Process Biochemistry


Bioprocesses based on the use of enzymes can be employed for the obtainment of ferulic acid by its solubilization from corn cob, which is a natural source of this phenolic compound. In order to overcome the limitations caused by the recalcitrance of biomass, the application of pretreatments is advisable. The utilization of high hydrostatic pressure on the enzymatic release of ferulic acid from corn cob with three feruloyl esterase extracts was studied applying pressurization at 600 MPa as a pretreatment in combination with a mild thermal treatment at 130 °C/2 h. Additionally, pressurization at 200 MPa during the enzymatic hydrolysis step was assessed to increase the ferulic acid yield. The results showed that the application of a high pressure pretreatment at 600 MPa/40 °C/15 min to thermally treated corn cob increased by 20% the ferulic acid release in comparison with the thermal treatment for Ultraflo® L, but not for the Aspergillus extracts. Pressurization at 200 MPa during the hydrolysis stage affected differently the enzymes stability and ferulic acid release depending on the extract and the pretreatments applied, showing that the effects of pressurization are complex and must be assessed in each case.

1-3-Dichloropropene 2030 Agenda priorities Abribone Particles Accumulation Acid Soils Acidification Activated Carbon Active ingredients Acuicultura Adsorption Adulteration Aeration Aerobiology Aggregation Agricultural Residue Agro-industrial waste Agronomic performance Agrostis capillaris Air temperature Airborne Alcohols Aldehyde Algae Alkaline Hydrolysis Alkylamines Allelochemical stress Allergy Aluminium Alzheimer's Disease Amendment Amino acid and purine biosynthesis Amphibolite Amylases Analysis Anionic Amphiphiles AnionsMetals ANN Anoma Anthocyanins Anti-aging Anti-cancer activity Antidepressant Antimicrobial Antimicrobial applications Antimicrobial compounds Antioxidants Antioxidants Activity Antiradical Activity Antitumorigenic activity Antiviral Apples Applications AQUA-CIBUS Aqueous solution Arabidopsis thaliana Arbequina ARIMA Aroma Aromatic compounds Aromatic plants Arsenic Artificial Neural Networks Ascorbic acid Ashes Atmospheric Pollution Authentication Autohydrolysis Auxins Availability Axisymetric Models Bacillus subtilis Bacterial growth Bacteriocin Bare fallow soils Barley straw Barrels Batch Beer Bentonite Berry Bilinear matrix Bioactive compounds Bioactive Food Components Bioactive substances Bioactivities Bioavailability Biochar Bioethanol Biofilm Biological indicator Biomarkers Biomonitoring Biorefinery Biosorbent Biotransformation Black Pepper Blockchain Technology Blueberry Body weight Boscalid Botrycides Botrytis cinerea Brassica juncea Brewery wastes Brown macroalgae BTEX Bullet corrosion Butter fat By-products Cadmium Caffeic Acid Calcium Candelilla Candidate gene Carbon isotope discrimination Carburan Catechin-rich extract Cattle Slurry Cell cycle Celta pig breed Characterization Cheese whey Chemical composition Chemical equilibrium Chemometrics Chemoresistance Chestnuts Chloropicrin Chlorteracycline Cholinesterases Chorizo Chromatography Chromium Circular economy Clasification Climate change Climate impact CMC Cobalt Colloids Color Column experiments Competitive sorption Compost Compounds Conformational changes Conjoint analysis Continuous fermentation Control Controlled deficit irrigation Cooking methods Copper Corn breeding Corn cob Corn stover Cornicabra Cortical Neurons Corticosteroids Corticosterone Cortisol Cosmetics Cow Milk Crop Protection Crushed Mussel Shell cucurbit[7]uril cucurbituril Curing Cycas pectinata Cyclic voltammetry Cyclodextrins Cyclopentadecanone Cytotoxicity Dactylis glomerata Data Acquisition and Management Data analysis Decomposition Decontamination Degradation Dehydrogenase activity Denitrosation Density Desorption DFT calculations Diabetes mellitus Dietary polyphenols Dinamic Surface Tension Discharge prediction Disease prevention Dissipation Dithiocarbamates Doxycycline Dry fruit Dry-cured Drying DTCs Edible films Edible flowers Ehrlich pathway Encapsulation Enrichment factors Enterococcus faecium Enzymatic hydrolysis Enzymatic saccharification ErbB2 Ergosterol biosynthesis Esencial Oil Essential oils Ethanol Ethnobotanic Ethylene Ethylenethiourea Eucalyptus camaldulensis EVOO EVOO applications EVOO quality EVOOs Extra Virgin Olive Oil Extraction Extraction Optimization Extraction techniques Fast growing biomass Fat healthiness Fat oxidation Fatty Acids Faults FE-SEM/EDS Feathers Fed-batch fermentation Fed-batch SSF Fed-batch system Feed intake Feluric Acid Feluroyl esterase Fenhexamid Ferhexamid Fermentation Ferulic acid Feruloyl Feruloyl esterase Fingerprint Firing range soils Fish oil Flavanols Flavor Flowering delay Fluorescence Fluoride Fluorine Focus group Folin-Ciocalteuassay Food additives Food analysis Food authentication Food Authenticity Food by-products Food composition Food fingerprinting Food intake-related public risks Food Quality Food Supply Chain Food sustainability Food systems Food Traceability Foods Forest Forest Soils Formación Fortification Fortified Wines Fourier transform infrared Fractionation Fraxinus Frog Frozen storage Frugal-innovation Fucoxanthin Fuidized bed reactor Fullerene Functional Enzymes Functional Food Fungal Spores Fungicide Fungicides Furfural Galicia Galician virgin olive oils Garlic Garnacha Tintorera Gastrointestinal tract GC GC/MS Gelation Generic diversity Genetic variation Geothermal systems Germination Germplasm charaterization Glassy network Glucomannan Gold nanoparticles Gourmet Graciano Granite Granite powder Granitic Material Grape juice Grapes Grasshopper Effect Gravitropism Green synthesis Growth Guava HAE Hair Health benefits Heat-Assited Extraction Heavy Metals Helath claim Hemicelluloses Hemp waste Heterocyclic aromatic amines High hydrostatic pressure High pressure High solids loading Histeresys index Histolocalization Hordeum vulgar HPLC HPLC-DAD HPLC-FLD HPLC-MS/MS HPLC/MS HR-TEM/EDS Humid acid Hyaluronic acid Hydrogeology Hydrolysis Hydroxycinnamic acid Hydroxylpropyl-beta-cyclodextrins Hyperspectral imaging immobilization In vitro Industrial applications Industrial level Innovative functional foods Instrumental analysis Ionic Liquids Iron Job Opportunities Jornadas Kale Keroxim Kinematic viscosity Kinetics Konjac glucomannan Laboratory column Lactic acid Lactic acid bacteria Land use LDT Lead Leaf water relations Lenga temperate forests Liberation Lignin Ligustrum Lime pretreatment Linear Discriminant Analysis Lipid oxidation Lipolysis Lipoxygenase Liqueurs Long-term fertilization Lotka-Volterra Low toxicity Maceration Machine learning Macroalgae Macroalgae applications Magnesium Maize populations Major Depressive Disorder MALDI-TOF/TOF-MS Mancozeb MAO Marcozeb Marinades Measures and indicators Meat Meat Quality Medicinal plant Mediterranean diet Membrane filtration Mepanipyrim Mercury Metabolism Metabolite Metabolites Metabolomics Metal Metal availability Metal fractionation Metalaxyl Meteorology Methyl isothiocyanate Metrafenone Micelles Microalgae Microbiota Microemulsions Microscopy Microtubules Mine Mine soil Mine tailing Mode of Action Modelisation Molecular docking Monoamine Oxidase MS MS/MS Multidrug resistance Multiple chemicals Multiproduct biorefinery Multivariate analysis Muscle foods Mussel Mussel shell Mustard plants Nanocoating Nanoparticle Nanowhisker NAO index Natural Colorants Natural sources Neonates Neonicitinoids Neurodegenerative Disorders Neuroprotection Neurotoxins Nickel NIR Nisin Nitric Oxide Nitrosomercaptopyridine NO Non-linear processes Noticias Novel technologies NPK Fertilizers Nutraceulticals Nutrients Oak ash Oak species Oat straw OAV Ocimum basilicum var. purpurascens leaves OCPs Odorants Odour Activity Value Ole e 1 Olea Oleaceae Olive Co-crushing Olive Oil Olive oil by-products OncomiR OPEs OPPs OPs Optimization Organic amendment Organic carbon Organic matter Organic pollutants Ourense Oxidation Oxidative damage Oxidative phase Oximes Oxytetracycline Ozone p-hydroxybenzoic acid PAHs Paper Industry Parkinson's Disease Pastureland Soils Pathogenic bacteria PBDEs PBDs PCBs PCDDs PCDFs PDO Pellets Percolation Perlite waste addition Pest Management Protocols Pesticide Pets pH pH-spectra Phenolic and aromatic compounds Phenolic Compounds Phenolics Phenology Phenotyping Phosphate Phosphorus Phosphorus adsorption Phosphorus desorption Physical protection Physiological responses Phytochemicals Phytopigments Phytostabilization Phytotoxic effects Phytotoxicity Picual Pig Pig genotypes Pig Stress Pigeon Piglets Pine bark Pine Sawdust Pinus sylvestris Pistachia vera Placenta Plant cell walls Plant production Plantago Plasma Pollen Polluted Soils Polluted Water Pollution Polymer Polyphenols Post-harvest drying Potato Prairie Prebiotic activity Precipitation Prediction Prenatal Preservation Prevention Probiotics Process optimization Production Profiling Properties Proteases protected denomination of origin Proteome profile Proteomics Public health Purification Putative transcription factors Pyritic material PYRs Quality-related Indices Quantification Racked bed reactor Rain Rainfastness Raman Random forest Rank annihilation factor analysis Raw Fish Oil Reaction kinetics modelling Reactor Realkalization Red Rubin Basil Red Wines Redes de Investigación Reinforced Wines Remediation Residues Resistance Response surface methodology Retaining capacity Retention Reuse Rheological properties Ripening temperature RISEGAL Risk assessment Risk Periodos River River bed sediments RMN Root growth Roots Rosaceae family RSM S-nitrosothiol Salting intensity Saponification Screening methods SDS SDS-PAGE Secondary Metabolites Seedling Seminarios Sensory analysis Sensory attributes Sepia Ink Sequential extraction Serotonin Sesamia nonagrioides Settling pond Shelf-life Shelterwood-cut silvicultural system Shooting range Short chain fatty acids Signalling pathways Silage Single extraction Slaughterhouse Large Time Soil Soil aggregates Soil Amendment Soil impact Soil pollution Soil remediation Soil residues Soil structure Soils Solanum tuberosum Solid-state fermentation Solvent Extraction Sorption Soybean oil Spectrometry Spectroscopy Speed of Sound Spirits SSR markers Stability Stress response Styrene Subcutaneous ham fat Subcutaneous pig back-fat Sulfamethoxazole Sulfur-containing compounds Support vector machine Surface Surface Tension Surfactants Sustainability Sustainable Adsorbents Sustainable Development Sustainable use of natural resources SWAdSV Sweet Wines Swertia chirata Taladros Tarbush Taste Technosol Tempranillo Tenacity Terra preta do índio Tetraciclyne Thermal gelation profiles Thermal springs Throughfall Tiamulin TOF-SIMS Toro Appellation of Origin Total aliphatic hydrocarbons Toxic cocktail effects Toxicity Traditional Smoked Foods Traditionally used plants trans-Caryophyllene Transcriptomics Transport Transport experiment Tree vegetation Trimethoprim Tropical soils Tumor suppressor miR Underground waters University of Vigo Urine UV-Vis spectroscopy Vaccinium corymbosum Vacuum packaging Valorization Vanadium Variedades tolerantes Vinclozolin Vine trimming shoot Vineyard Virgin Olive Oils Virus Viscosity Vitamin Volatile Volatile Compounds Voltímetro Wash-off Waste reduction Wastes Wastewater Water deficit Water efficiency Water pollution Water quality Water temperature Weakly deacetylation Weather Webinar Wells Wheat straw Whey Wine Wine aging Wine Quality Wine-making Practices Winemaking Process Withered inflorescences Wood Wood Ash Xylitol Xylooligosaccharides Xylose Zea mays Zinc β-Lactoglobulin